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Good News Everyone - Researchers creating Mutant Ebola Viruses

 
Fhirinne
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User ID: 1063055
United Kingdom
03/21/2012 07:17 PM
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Good News Everyone - Researchers creating Mutant Ebola Viruses
Reverse Genetics Approach for Generating Ebola Virus and Other Filoviruses from Cloned DNA

Kawaoka and his colleagues have now developed a reverse genetics approach for generating Ebola virus entirely from cloned cDNA. They prepared the full Ebola genome through reverse transcription of viral RNA,

The researchers also used the system to make mutant virus particles

[link to www.warf.org]

I see from the guys profile he gets funding from the Gates Foundation not for Ebola like but well I'd say they certainly have an interest in it with this story from back in 2009 “Gates Foundation hires discoverer of Ebola”
[link to www.usatoday.com]



[link to jvi.asm.org]

ABSTRACT

Ebola virus, a prime example of an emerging pathogen, causes fatal hemorrhagic fever in humans and in nonhuman primates. Identification of major determinants of Ebola virus pathogenicity has been hampered by the lack of effective strategies for experimental mutagenesis. Here we exploit a reverse genetics system that allows the generation of Ebola virus from cloned cDNA to engineer a mutant Ebola virus with an altered furin recognition motif in the glycoprotein (GP). When expressed in cells, the GP of the wild type, but not of the mutant, virus was cleaved into GP1 and GP2. Although posttranslational furin-mediated cleavage of GP was thought to be an essential step in Ebola virus infection, generation of a viable mutant Ebola virus lacking a furin recognition motif in the GP cleavage site demonstrates that GP cleavage is not essential for replication of Ebola virus in cell culture.
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While studying virus is it really necessary to mutate the crap into something that’s "one of the most lethal human pathogens recognized to date?" Before we know it there is a slowed down version of Ebola sat in a Petri dish or test tube that might as well be called E-SHTF...
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monsterrun

User ID: 19129594
Canada
07/30/2012 03:59 PM
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Re: Good News Everyone - Researchers creating Mutant Ebola Viruses

To live is to believe in the power of dreams! To dream is to believe in the power of love! To love is to Know the truth!
The Desire to Be fuel the belief that you Are which ignite the Will to Become which bring back forth the desire to be...
Let it be-come you! It means Stop seeking your higher self! It is seeking you! Stand still in your mind to calm the waters of your mind and then it shall find you, so you can ride those waves together!
your true self lies somewhere between your heart and your consciousness. It is called the heart consciousness,which is the creator, which is you!
The heart create the emotions and our mind evoke its purpose, from which we dream the life we live in order to imagine the nature of reality and finally remember love!

The highest Purpose of our mind is the ability to Forget! Go on and Forgive yourself!
There is no love in truth but there is truth in love!
Be authentic, nobody else can do it for you!

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