Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" | |
Anonymous Coward User ID: 61651729 United States 10/02/2014 03:36 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" So Bill Gates gives $50 million to ebola "research". Quoting: Anonymous Coward 20413302 He should have written the check directly to the airlines and asked them TO STOP FLYING TO FREAKING EBOLA TERRITORIES!!!! He's on bloomberg right now being interviewed, an exclusive interview. Guess he's off the yacht and back on land. I wish he'd stop using the soup bowl to cut his hair. |
Nobuddy User ID: 56312542 Canada 10/02/2014 03:37 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Ever notice how lone gunmen become historically known by their first and last names.. serial killers become known historically by first middle and last names. Thomas Eric Duncan should be remembered from this day forward as the first documented walking Weapon of Mass Destruction. He knowingly exposed himself & purposely timed his deployment to the US. He also ensured that he purposely exposed as many people as he could once he got here. Including his own family members, allowing them out into the community ensuring maximum exposure to healthcare workers, first responders, educators, children & now agency representatives alike. This has all the earmarks of an intentional act.. we need to stop referring to 'Thomas Eric Duncan' as a victim. He's become a self-replicating weapon of mass destruction & he needs to be treated as such. |
Dr. Astro Senior Forum Moderator User ID: 4211721 United States 10/02/2014 03:38 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Well the alternative is that you've got an agenda behind your false accusations. I'm actually avoiding attributing it to actual malice. An agenda? You mad? You actually serious here or what? False accusations and malice... You need to get a grip of reality. Learn how to quote properly. Thanks. I said I was assuming you didn't have an agenda... and that's your reaction? Now who's acting defensive? You. I do have a very firm grip on reality. Quote where I "made up stories." I didn't. It was a false accusation. I'm assuming it's just harmless trolling though. It's a shame, I actually agree with you about the siRNA cocktail, it's extremely promising as a treatment strategy. I'm far more grounded in reality than you give me credit for. Nowhere did I suggest that he was a threat to passengers prior to being symptomatic which seemed to be the thrust of your initial complaints around here. That you would feel the need to falsely accuse me of making stories up is disappointing. |
EscapeVelocity User ID: 19583375 South Korea 10/02/2014 03:38 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Do we really know that for sure? Or are we taking his word for it? He spent some 28 hours flying on various planes. That's a long damn time. Even if he wasn't presenting symptoms when he first boarded, even if he wasn't presenting when he passed customs in DC he still had a layover and connection to DFW. How sure can we be that he didn't start developing the first stage of symptoms somewhere along the way and simply lie about when his fever really started? He lied before, he can lie again. That's what worries me, how thorough is our screening once they arrive on our shores and start making connecting flights? Do we check them as they disembark at their FINAL destination somewhere deeper in the country like DFW? I'm asking because I honestly don't know the answer to that, I haven't seen it clearly delineated. Most first world nations use FLIR at customs checkpoint, you can see a subject with an abnormal body temperature before the approach the passport control officer's window and flag them for further evaluation. At least in Asia, we dont fuck around after bird flu. If you have a temp, you cant hide it, and the only "Africans" we get here are American service men and women, who I will add are quite welcome. Just ask any black man what it is like going through customs in Japan... No mercy, your shit is searched and your papers better be in order, unless your an American, European or Canadian passport holder. It seems you folks get endless security theater, with no actual security... No offense to anybody of color, but folks from Africa have different standards for hygiene, ethics and normalcy. They will be properly screened, regardless of hurt feelings. I remember a few months ago when I was in a EU airport, a well dressed African (obviously not poor) couples child was trying to reach over the seat in the terminal lounge and dig his hands into my bag of food on the seat next to me, I remarked to his mother that this is not appropriate, and she looked at me with a nasty expression. AFTER he succeeded in getting his little hand into the bag, I just gave him the food and left... he ate it. Have compassion, but understand... they play by different rules, rules that would be unthinkable and abhorrent to westerners (or Asians) |
Anonymous Coward User ID: 56681215 United States 10/02/2014 03:38 PM Report Abusive Post Report Copyright Violation | |
Anonymous Coward User ID: 63543894 United States 10/02/2014 03:39 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Well, in about two weeks we'll see if ebola is a majority of states. Quoting: Anonymous Coward 63378084 within 30 days we'll know if tptb are really behind his or not. if it is quelled and the 'fever pitch' dies down ...then it was just alot of stupidity mixed with paranoia. if several people have it in several cities... doom on. and it truly 'has begun'. |
Anonymous Coward User ID: 61266622 United States 10/02/2014 03:41 PM Report Abusive Post Report Copyright Violation | |
Anonymous Coward User ID: 56681215 United States 10/02/2014 03:44 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" "@GoldenStateEMS: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Quoting: Uncle Fuck Stick [link to abc7.com] ABC7 has learned new information about the United Airlines jetliners that brought the first American Ebola victim, Thomas Eric Duncan, into the United States. Among the findings: One of the planes that carried Duncan later made several stops at LAX. SEE THE FULL LIST OF FLIGHTS AND CITIES FOR THE TWO JETS AT THE BOTTOM OF THIS ARTICLE. When Duncan left Liberia, he made his way to Brussels and boarded a United plane for the U.S. On Sept. 20, he took a United Boeing 777 from Brussels to Washington Dulles, before clearing customs and taking his connecting flight, a United Airbus 320 from Washington to Dallas. Since then, based on their tail numbers, the two aircraft have carried passengers to 27 different cities, including Los Angeles. In fact, the A-320 landed in Los Angeles as recently as yesterday. "As Americans, we can and should be proud of the progress our country has made over these past six years." —President Obama [link to twitter.com (secure)] |
Dr. Astro Senior Forum Moderator User ID: 4211721 United States 10/02/2014 03:47 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Do we really know that for sure? Or are we taking his word for it? He spent some 28 hours flying on various planes. That's a long damn time. Even if he wasn't presenting symptoms when he first boarded, even if he wasn't presenting when he passed customs in DC he still had a layover and connection to DFW. How sure can we be that he didn't start developing the first stage of symptoms somewhere along the way and simply lie about when his fever really started? He lied before, he can lie again. That's what worries me, how thorough is our screening once they arrive on our shores and start making connecting flights? Do we check them as they disembark at their FINAL destination somewhere deeper in the country like DFW? I'm asking because I honestly don't know the answer to that, I haven't seen it clearly delineated. Most first world nations use FLIR at customs checkpoint, you can see a subject with an abnormal body temperature before the approach the passport control officer's window and flag them for further evaluation. That's why I'm asking though if we check them after they get past customs and onto their flights to their final destination further into the country. He had a three hour layover in DC plus the time it took to fly to Dallas. Do we scan them with FLIR at their final destination when they disembark? I'm guessing we don't (we ought to) but I don't know, so I'm asking. I'm not making up stories, I'm asking. Last Edited by Astromut on 10/02/2014 03:48 PM |
Anonymous Coward User ID: 44493546 United States 10/02/2014 03:47 PM Report Abusive Post Report Copyright Violation | |
Anonymous Coward User ID: 55544075 United Kingdom 10/02/2014 03:48 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Well the alternative is that you've got an agenda behind your false accusations. I'm actually avoiding attributing it to actual malice. An agenda? You mad? You actually serious here or what? False accusations and malice... You need to get a grip of reality. Learn how to quote properly. Thanks. I said I was assuming you didn't have an agenda... and that's your reaction? Now who's acting defensive? You. I do have a very firm grip on reality. Quote where I "made up stories." I didn't. It was a false accusation. I'm assuming it's just harmless trolling though. It's a shame, I actually agree with you about the siRNA cocktail, it's extremely promising as a treatment strategy. I'm far more grounded in reality than you give me credit for. Nowhere did I suggest that he was a threat to passengers prior to being symptomatic which seemed to be the thrust of your initial complaints around here. That you would feel the need to falsely accuse me of making stories up is disappointing. Then my apologies - 99% are insane here. It's hard to sift through, and maybe I misread your original post. I do know how to quote, but on that occasion I didn't, again apologies for that. Maybe we can move forward on this... siRNA... I have done quote extensive research myself on it. |
Anonymous Coward User ID: 61318070 Portugal 10/02/2014 03:50 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Ever notice how lone gunmen become historically known by their first and last names.. serial killers become known historically by first middle and last names. Quoting: Nobuddy 56312542 Thomas Eric Duncan should be remembered from this day forward as the first documented walking Weapon of Mass Destruction. He knowingly exposed himself & purposely timed his deployment to the US. He also ensured that he purposely exposed as many people as he could once he got here. Including his own family members, allowing them out into the community ensuring maximum exposure to healthcare workers, first responders, educators, children & now agency representatives alike. This has all the earmarks of an intentional act.. we need to stop referring to 'Thomas Eric Duncan' as a victim. He's become a self-replicating weapon of mass destruction & he needs to be treated as such. Good point sir. |
Anonymous Coward User ID: 62007420 United States 10/02/2014 03:51 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" "@GoldenStateEMS: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Quoting: Uncle Fuck Stick [link to abc7.com] ABC7 has learned new information about the United Airlines jetliners that brought the first American Ebola victim, Thomas Eric Duncan, into the United States. Among the findings: One of the planes that carried Duncan later made several stops at LAX. SEE THE FULL LIST OF FLIGHTS AND CITIES FOR THE TWO JETS AT THE BOTTOM OF THIS ARTICLE. When Duncan left Liberia, he made his way to Brussels and boarded a United plane for the U.S. On Sept. 20, he took a United Boeing 777 from Brussels to Washington Dulles, before clearing customs and taking his connecting flight, a United Airbus 320 from Washington to Dallas. Since then, based on their tail numbers, the two aircraft have carried passengers to 27 different cities, including Los Angeles. In fact, the A-320 landed in Los Angeles as recently as yesterday. FUCK. |
Dr. Astro Senior Forum Moderator User ID: 4211721 United States 10/02/2014 03:52 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ... Quoting: Dr. Astro Well the alternative is that you've got an agenda behind your false accusations. I'm actually avoiding attributing it to actual malice. An agenda? You mad? You actually serious here or what? False accusations and malice... You need to get a grip of reality. Learn how to quote properly. Thanks. I said I was assuming you didn't have an agenda... and that's your reaction? Now who's acting defensive? You. I do have a very firm grip on reality. Quote where I "made up stories." I didn't. It was a false accusation. I'm assuming it's just harmless trolling though. It's a shame, I actually agree with you about the siRNA cocktail, it's extremely promising as a treatment strategy. I'm far more grounded in reality than you give me credit for. Nowhere did I suggest that he was a threat to passengers prior to being symptomatic which seemed to be the thrust of your initial complaints around here. That you would feel the need to falsely accuse me of making stories up is disappointing. Then my apologies - 99% are insane here. It's hard to sift through, and maybe I misread your original post. I do know how to quote, but on that occasion I didn't, again apologies for that. Maybe we can move forward on this... siRNA... I have done quote extensive research myself on it. Do you have a detailed protocol available for the process of encapsulating the siRNA cocktail stably into the nucleic acid lipid particles? I have a general protocol of how to do it with a peristaltic pump, and they list the lipids they used in the journal article about the non-human primate experiment, but have you happened to come across a more specific and detailed protocol of the process they used? |
Anonymous Coward User ID: 61318070 Portugal 10/02/2014 03:54 PM Report Abusive Post Report Copyright Violation | |
EscapeVelocity User ID: 19583375 South Korea 10/02/2014 03:54 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" Do we really know that for sure? Or are we taking his word for it? He spent some 28 hours flying on various planes. That's a long damn time. Even if he wasn't presenting symptoms when he first boarded, even if he wasn't presenting when he passed customs in DC he still had a layover and connection to DFW. How sure can we be that he didn't start developing the first stage of symptoms somewhere along the way and simply lie about when his fever really started? He lied before, he can lie again. That's what worries me, how thorough is our screening once they arrive on our shores and start making connecting flights? Do we check them as they disembark at their FINAL destination somewhere deeper in the country like DFW? I'm asking because I honestly don't know the answer to that, I haven't seen it clearly delineated. Most first world nations use FLIR at customs checkpoint, you can see a subject with an abnormal body temperature before the approach the passport control officer's window and flag them for further evaluation. That's why I'm asking though if we check them after they get past customs and onto their flights to their final destination further into the country. He had a three hour layover in DC plus the time it took to fly to Dallas. Do we scan them with FLIR at their final destination when they disembark? I'm guessing we don't (we ought to) but I don't know, so I'm asking. I'm not making up stories, I'm asking. Sorry Astro, I can only speak to the passport control standards in first world Asia (Japan,Korea etc.) As for the states, your TSA is just as likely to spread infections by reusing examination gloves and stopping passengers carrying cash instruments then controlling the spread of infectious diseases. As I said, security theater. |
Mr BubbaHyde User ID: 49117730 United States 10/02/2014 03:55 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" does anyone realize that the ebola virus, when not inside a persons warm body, dies, almost immediately......geez.....what's the big deal??? Quoting: Anonymous Coward 55109471 Oh Boy, Ok How I occupy my spare time... [link to www.youtube.com (secure)] [link to open.spotify.com (secure)] |
Nobuddy User ID: 56312542 Canada 10/02/2014 04:04 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ..& now it looks like the family has no regard for public safety & intentionally violated the voluntary quarantine. The numbers will probably jump to 2-300 people who may have been exposed.. of those people how many are going to run 'full-retard' into crowded medical facilities & expose even more people. The 'Duncan' bomb has detonated & Dallas is now ground zero.. |
Anonymous Coward User ID: 55544075 United Kingdom 10/02/2014 04:06 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ... Quoting: Anonymous Coward 55544075 An agenda? You mad? You actually serious here or what? False accusations and malice... You need to get a grip of reality. Learn how to quote properly. Thanks. I said I was assuming you didn't have an agenda... and that's your reaction? Now who's acting defensive? You. I do have a very firm grip on reality. Quote where I "made up stories." I didn't. It was a false accusation. I'm assuming it's just harmless trolling though. It's a shame, I actually agree with you about the siRNA cocktail, it's extremely promising as a treatment strategy. I'm far more grounded in reality than you give me credit for. Nowhere did I suggest that he was a threat to passengers prior to being symptomatic which seemed to be the thrust of your initial complaints around here. That you would feel the need to falsely accuse me of making stories up is disappointing. Then my apologies - 99% are insane here. It's hard to sift through, and maybe I misread your original post. I do know how to quote, but on that occasion I didn't, again apologies for that. Maybe we can move forward on this... siRNA... I have done quote extensive research myself on it. Do you have a detailed protocol available for the process of encapsulating the siRNA cocktail stably into the nucleic acid lipid particles? I have a general protocol of how to do it with a peristaltic pump, and they list the lipids they used in the journal article about the non-human primate experiment, but have you happened to come across a more specific and detailed protocol of the process they used? Well you surprise me, I just thought you was into Astronomy! Done some Virology too? Anyways, the answer to your question is Yes. The process used is by a liposome vesicle which as you know can be filled with drugs, but it's with this process that you can form a nucleic-acid-lipid particle, or SNALP, it's much better using siRNA-SNALP than using it unformulated. It will then be injected intravenously. |
Anonymous Coward User ID: 63532478 United States 10/02/2014 04:09 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ... Quoting: Anonymous Coward 2920706 You're a virologist with specific concentration on ebolavirus to know this right? You have years of experience that says no single ebola strain can ever be contagious in a host until fever and symptoms hit some voodoo X level of fever, vomiting, and diarrhea, yes? Or did you just read a news story telling you that...... Exactly. Told to us by the same people who said the fundamentals of the economy were strong in 2008, and Iraq had WMDs, and building 7 collapsed symmetrically at free-fall speed from structure fire, and the Gulf of Tonkin happened, and Iraqis threw babies from incubators, etc. Are you attempting to win dumbass of the year award? The Government does not tell you this, god damn world renowned scientists do. FFS jumped up tard. You're such a fucking moron. We need to start banning perma banning retards like you. The ridiculous assumption that you are clinging to is why the virus is out of control in the first place because few are taking the necessary precautions to stop it. Ebola is airborne on micro droplets of water you exhale from your lungs, you know how your breath produces fog when its cold? That's water (bodily fluid) you tard and the WHO has even confirmed that it's spread this way while delusional "professionals" who mostly reside in the US cling to the notion that it can't be spread that way. Let's not forget the virus also can spread through sweat, which has been theorized by both the CDC and WHO. The assumption that disease can't be spread until you are symptomatic is as big of a fucking lie as it gets, and is being pedaled by our media and their "experts" far more than actual virologists and health organizations. Our media and their "experts" are clinging to the non infectious incubation period based not on scientific knowledge but because they assume that since because people aren't shitting puking and bleeding all over the place that it can't be spread during the incubation period. The WHO came right out and called it fake. But they cleverly spelled it "PHEIC," so people such as yourself would keep believing. Bless your heart. |
Anonymous Coward User ID: 55544075 United Kingdom 10/02/2014 04:09 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" I do wish though that these studies into siRNA would have got more funding & publicity than it does. Although with the latest Ebola outbreak at the moment, I believe this will be heavily studied. If I was a betting man, i'd be buying shares into Tekmira right about now. |
Dr. Astro Senior Forum Moderator User ID: 4211721 United States 10/02/2014 04:14 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ... Quoting: Dr. Astro Learn how to quote properly. Thanks. I said I was assuming you didn't have an agenda... and that's your reaction? Now who's acting defensive? You. I do have a very firm grip on reality. Quote where I "made up stories." I didn't. It was a false accusation. I'm assuming it's just harmless trolling though. It's a shame, I actually agree with you about the siRNA cocktail, it's extremely promising as a treatment strategy. I'm far more grounded in reality than you give me credit for. Nowhere did I suggest that he was a threat to passengers prior to being symptomatic which seemed to be the thrust of your initial complaints around here. That you would feel the need to falsely accuse me of making stories up is disappointing. Then my apologies - 99% are insane here. It's hard to sift through, and maybe I misread your original post. I do know how to quote, but on that occasion I didn't, again apologies for that. Maybe we can move forward on this... siRNA... I have done quote extensive research myself on it. Do you have a detailed protocol available for the process of encapsulating the siRNA cocktail stably into the nucleic acid lipid particles? I have a general protocol of how to do it with a peristaltic pump, and they list the lipids they used in the journal article about the non-human primate experiment, but have you happened to come across a more specific and detailed protocol of the process they used? Well you surprise me, I just thought you was into Astronomy! Done some Virology too? Astronomy is my hobby. I'm not a virologist, but let's just say I've done my fair share of RNA work (gene expression studies), though not involving siRNA specifically. Anyways, the answer to your question is Yes. The process used is by a liposome vesicle which as you know can be filled with drugs, but it's with this process that you can form a nucleic-acid-lipid particle, or SNALP, it's much better using siRNA-SNALP than using it unformulated. It will then be injected intravenously. Quoting: ACI have a fair degree of understanding of the overall process, but I guess I was hoping for more detail on the lipid concentrations used to create the vesicles, and the details on the exact concentrations and timing used in the process to encapsulate the siRNA within the SNALPs. |
Anonymous Coward User ID: 56550270 United States 10/02/2014 04:14 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" how is it that all flights and customers are tracked by computers and this guy's itinerary is so cloudy? Quoting: Anonymous Coward 63534062 Because "They" want it that way. Yes...^^^This. They can chemtrail it from the air if they wanted to...but airport travel keeps people terrified of going out, blaming and turning on each other and glued to their programming devices for further instruction. They get off on that kind of stuff..... |
Dr. Astro Senior Forum Moderator User ID: 4211721 United States 10/02/2014 04:16 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" @ Dr. Astro Quoting: Anonymous Coward 55544075 I do wish though that these studies into siRNA would have got more funding & publicity than it does. Although with the latest Ebola outbreak at the moment, I believe this will be heavily studied. If I was a betting man, i'd be buying shares into Tekmira right about now. I'm kicking myself for not transfering some of my investments into Tekmira earlier. I understand they're currently competing for a grant to take their drug into trials in west Africa right now. No guarantee they'll win, but their stock price is already going up. |
Anonymous Coward User ID: 6524535 Canada 10/02/2014 04:17 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" does anyone realize that the ebola virus, when not inside a persons warm body, dies, almost immediately......geez.....what's the big deal??? Quoting: Anonymous Coward 55109471 It's been believed that the bacteria can survive for a while outside the body. Many lesser harmful diseases can do that as well. Some viruses can sustain through moisture for a few secs/mins. |
Anonymous Coward User ID: 55544075 United Kingdom 10/02/2014 04:20 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" does anyone realize that the ebola virus, when not inside a persons warm body, dies, almost immediately......geez.....what's the big deal??? Quoting: Anonymous Coward 55109471 It's been believed that the bacteria can survive for a while outside the body. Many lesser harmful diseases can do that as well. Some viruses can sustain through moisture for a few secs/mins. OP of the quote is not telling the truth. Ebola has been proven to live up to 50 days outside of the host. |
Anonymous Coward User ID: 55544075 United Kingdom 10/02/2014 04:24 PM Report Abusive Post Report Copyright Violation | Re: Jet that carried #Ebola victim later landed at #LosAngeles #Airport. This is as serious as it gets!!" ... Quoting: Anonymous Coward 55544075 Then my apologies - 99% are insane here. It's hard to sift through, and maybe I misread your original post. I do know how to quote, but on that occasion I didn't, again apologies for that. Maybe we can move forward on this... siRNA... I have done quote extensive research myself on it. Do you have a detailed protocol available for the process of encapsulating the siRNA cocktail stably into the nucleic acid lipid particles? I have a general protocol of how to do it with a peristaltic pump, and they list the lipids they used in the journal article about the non-human primate experiment, but have you happened to come across a more specific and detailed protocol of the process they used? Well you surprise me, I just thought you was into Astronomy! Done some Virology too? Astronomy is my hobby. I'm not a virologist, but let's just say I've done my fair share of RNA work (gene expression studies), though not involving siRNA specifically. Anyways, the answer to your question is Yes. The process used is by a liposome vesicle which as you know can be filled with drugs, but it's with this process that you can form a nucleic-acid-lipid particle, or SNALP, it's much better using siRNA-SNALP than using it unformulated. It will then be injected intravenously. Quoting: ACI have a fair degree of understanding of the overall process, but I guess I was hoping for more detail on the lipid concentrations used to create the vesicles, and the details on the exact concentrations and timing used in the process to encapsulate the siRNA within the SNALPs. I have my papers on it from Uni still. (Not called med school in UK) - i'd love to share, it's all cited too. |
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