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Subject Found a Moderna patent from 2014. Luciferase mentioned 55 times & Luciferin - 5 times. PLUS, J&J and Regeneron Monoclonal Antibodies.

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[quote:Anonymous Coward 80828595:MV80OTIyNTY2Xzg5OTU5Nzg4X0YzRjYwMDZE]
[b]Preparation and Injection of Luciferin for
In Vivo Bioluminescent Assays
[/b]
Materials needed:
D-Luciferin, Firefly, potassium salt, 1.0 g/vial, (Xenogen Catalog #XR-1001) DPBS, w/o Mg2+ and Ca2+
Note: One can either reconstitute the entire 1.0 g of [b][color=red]D-Luciferin in 66.6 ml[/color] [/b]of DPBS or reconstitute the quan- tity of needed for an individual experiment.

From the following website:

https://www.ohsu.edu/sites/default/files/2019-04/Luciferin-Detection.pdf

and this.....

3. How many mice does one luciferin stock solution provide for?

Our stock solution recipe makes a 15 mg/ml solution using [b][color=red]1 gram of luciferin and 66.6 ml of DPBS[/color][/b]. Total volume of stock solution shouldn’t change dramatically, [b][color=red]so we’ll maintain the 66.6 ml in our math.[/color][/b]

In a 20 g mouse, use 200 µl luciferin stock solution.
[b][color=red]66.6 ml yields a total volume of 66,600 µl.
66,600 µl / 200 µl = 333 mice.[/color][/b]

From the following website:

https://www.goldbio.com/articles/article/in-vivo-luciferin-faqs
[/quote]

Original Message In the patent is the following: (Input 66.6 in the find feature)

To create luciferin, 1g of D-luciferin potassium or Sodium salt was dissolved in 66.6ml of distilled phosphate buffer solution (DPBS), not containing Mg2+ or Ca2+, to makea 15mg/ml Solution.

Here's the link:

[link to patentimages.storage.googleapis.com (secure)]

This has been planned for a long time.

Pictures (click to insert)





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