Users Online Now:
1,705
(
Who's On?
)
Visitors Today:
66,508
Pageviews Today:
138,429
Threads Today:
78
Posts Today:
1,127
01:28 AM
Directory
Adv. Search
Topics
Forum
Back to Forum
Back to Thread
REPLY TO THREAD
Subject
Found a Moderna patent from 2014. Luciferase mentioned 55 times & Luciferin - 5 times. PLUS, J&J and Regeneron Monoclonal Antibodies.
User Name
Font color:
Default
Dark Red
Red
Orange
Brown
Yellow
Green
Olive
Cyan
Blue
Dark Blue
Indigo
Violet
Black
Font:
Default
Verdana
Tahoma
Ms Sans Serif
In accordance with industry accepted best practices we ask that users limit their copy / paste of copyrighted material to the relevant portions of the article you wish to discuss and no more than 50% of the source material, provide a link back to the original article and provide your original comments / criticism in your post with the article.
[quote:Anonymous Coward 80828595:MV80OTIyNTY2Xzg5OTU5Nzg4X0YzRjYwMDZE] [b]Preparation and Injection of Luciferin for In Vivo Bioluminescent Assays [/b] Materials needed: D-Luciferin, Firefly, potassium salt, 1.0 g/vial, (Xenogen Catalog #XR-1001) DPBS, w/o Mg2+ and Ca2+ Note: One can either reconstitute the entire 1.0 g of [b][color=red]D-Luciferin in 66.6 ml[/color] [/b]of DPBS or reconstitute the quan- tity of needed for an individual experiment. From the following website: https://www.ohsu.edu/sites/default/files/2019-04/Luciferin-Detection.pdf and this..... 3. How many mice does one luciferin stock solution provide for? Our stock solution recipe makes a 15 mg/ml solution using [b][color=red]1 gram of luciferin and 66.6 ml of DPBS[/color][/b]. Total volume of stock solution shouldn’t change dramatically, [b][color=red]so we’ll maintain the 66.6 ml in our math.[/color][/b] In a 20 g mouse, use 200 µl luciferin stock solution. [b][color=red]66.6 ml yields a total volume of 66,600 µl. 66,600 µl / 200 µl = 333 mice.[/color][/b] From the following website: https://www.goldbio.com/articles/article/in-vivo-luciferin-faqs [/quote]
Original Message
In the patent is the following: (Input 66.6 in the find feature)
To create luciferin, 1g of D-luciferin potassium or Sodium salt was dissolved in 66.6ml of distilled phosphate buffer solution (DPBS), not containing Mg2+ or Ca2+, to makea 15mg/ml Solution.
Here's the link:
[
link to patentimages.storage.googleapis.com (secure)
]
This has been planned for a long time.
Pictures (click to insert)
General
Politics
Bananas
People
Potentially Offensive
Emotions
Big Round Smilies
Aliens and Space
Friendship & Love
Textual
Doom
Misc Small Smilies
Religion
Love
Random
View All Categories
|
Next Page >>