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Going Viral-Ebola vaccine is having ‘major impact’ but Congo outbreak may still explode; pg12
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Feistylorax |
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Nawty, Feisty, I want to hear from you two on this...Please respond with comments when you get back...Article on common testing methods for the virus and potential for false positive results, even with genetic strains we are aware of yet alone variations from pre-existing strains.... [ link to www.nature.com (secure)] ELISA-based screening assays, although efficient, can yield high false positive and false negative rates compared with functional seroneutralization (SN) assays19. Thus, whenever possible, ELISA/Luminex-positive samples are confirmed by a SN assay. Although SN assays are considered a gold standard for seroprevalence studies19,20,21, follow-up confirmation with live virus SN assays is limited by the amount of sample available, and the requirement to work with live HNV in a high-containment facility (BSL-4). Consequently, in many prior studies only ELISA/Luminex-positive samples, and often only a small subset such as those with the highest binding activity, were confirmed with a biological or surrogate SN assay (reviewed in LF Wang et al.21; for example, AJ Peel et al.22). The latter is based on serum antibody competition of soluble receptor (sEphrinB2-Fc) binding to recombinant NiV-G or HeV-G conjugated to Luminex beads18. Although these procedures can guard against false positives, they do not address the loss of potential false negatives10,13,14. Reading and noting With a 3-4% sero positive rate in those who are handling fruit bats with no reported cases to the point where they didn't know it is in africa is concerning, what else are they freaking missing, So it looks like in their testing they found a way to workaround the BSL 4 live virus specifications, Though I didn't see them finding a way to test how effective that system is for false negatives and positives in comparison. My summary would be that I think this may make things a bit more complex. If we have that much crossover in them they would be able to switch and transfer genes moderately well. Cross over events aren't even able to be mapped out yet since most of the strain variants aren't in the system. Not sure if that answers what you want, this article is pretty much a pointing out of how little they know about these viruses compared to what they need to know. Edit* just realized that I had not finished the report, I will try to get back to finish it later, but I've a crying baby and 2 to feed lunch to so no promises. It looks like they did do a test for effectiveness in a BSL4 so that previous statement was wrong.
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